Review





Similar Products

gene ruler 1 kb plus dna ladder  (Thermo Fisher)
98
Thermo Fisher gene ruler 1 kb plus dna ladder
(A–C) DNA sequencing chromatogram peak analysis, indicating that the genotype at rs1024611 in THP-1 cells was successfully converted from G/A (A) to G/G (B) and A/A (C). (D) PCR-amplified 321 bp fragments spanning the SNP site were digested with PvuII to distinguish parental and edited alleles. Parental cells (G/A heterozygous) show three bands of 321, 182, and 139 bp, reflecting the presence of both alleles. G/G mutant clones display two bands of 182 and 139 bp, indicating homozygosity for the G allele. A/A mutant clones show a single 321 bp band, consistent with homozygosity for the A allele. This restriction digest confirms the genotypes of the edited THP-1 clones. The GeneRuler 1 kb Plus <t>DNA</t> <t>Ladder</t> was used as a molecular size marker. The chromatograms shown in Figure 2 were generated and analyzed using Chromas version 2.6.6 software or Geneious R8 version.
Gene Ruler 1 Kb Plus Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+ruler+1+kb+dna+ladder/pmc13103971-81-0-7?v=Thermo+Fisher
Average 98 stars, based on 1 article reviews
gene ruler 1 kb plus dna ladder - by Bioz Stars, 2026-07
98/100 stars
  Buy from Supplier
gene ruler 1 kb dna ladder  (Thermo Fisher)
96
Thermo Fisher gene ruler 1 kb dna ladder
(A–C) DNA sequencing chromatogram peak analysis, indicating that the genotype at rs1024611 in THP-1 cells was successfully converted from G/A (A) to G/G (B) and A/A (C). (D) PCR-amplified 321 bp fragments spanning the SNP site were digested with PvuII to distinguish parental and edited alleles. Parental cells (G/A heterozygous) show three bands of 321, 182, and 139 bp, reflecting the presence of both alleles. G/G mutant clones display two bands of 182 and 139 bp, indicating homozygosity for the G allele. A/A mutant clones show a single 321 bp band, consistent with homozygosity for the A allele. This restriction digest confirms the genotypes of the edited THP-1 clones. The GeneRuler 1 kb Plus <t>DNA</t> <t>Ladder</t> was used as a molecular size marker. The chromatograms shown in Figure 2 were generated and analyzed using Chromas version 2.6.6 software or Geneious R8 version.
Gene Ruler 1 Kb Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+ruler+1+kb+dna+ladder/pmc12868784-272-0-8?v=Thermo+Fisher
Average 96 stars, based on 1 article reviews
gene ruler 1 kb dna ladder - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier

Image Search Results


(A–C) DNA sequencing chromatogram peak analysis, indicating that the genotype at rs1024611 in THP-1 cells was successfully converted from G/A (A) to G/G (B) and A/A (C). (D) PCR-amplified 321 bp fragments spanning the SNP site were digested with PvuII to distinguish parental and edited alleles. Parental cells (G/A heterozygous) show three bands of 321, 182, and 139 bp, reflecting the presence of both alleles. G/G mutant clones display two bands of 182 and 139 bp, indicating homozygosity for the G allele. A/A mutant clones show a single 321 bp band, consistent with homozygosity for the A allele. This restriction digest confirms the genotypes of the edited THP-1 clones. The GeneRuler 1 kb Plus DNA Ladder was used as a molecular size marker. The chromatograms shown in Figure 2 were generated and analyzed using Chromas version 2.6.6 software or Geneious R8 version.

Journal: Bio-protocol

Article Title: Protocol for Using CRISPR-Cas9 to Generate a Monocyte Cell Line Harboring a Single-Nucleotide Polymorphism

doi: 10.21769/BioProtoc.5660

Figure Lengend Snippet: (A–C) DNA sequencing chromatogram peak analysis, indicating that the genotype at rs1024611 in THP-1 cells was successfully converted from G/A (A) to G/G (B) and A/A (C). (D) PCR-amplified 321 bp fragments spanning the SNP site were digested with PvuII to distinguish parental and edited alleles. Parental cells (G/A heterozygous) show three bands of 321, 182, and 139 bp, reflecting the presence of both alleles. G/G mutant clones display two bands of 182 and 139 bp, indicating homozygosity for the G allele. A/A mutant clones show a single 321 bp band, consistent with homozygosity for the A allele. This restriction digest confirms the genotypes of the edited THP-1 clones. The GeneRuler 1 kb Plus DNA Ladder was used as a molecular size marker. The chromatograms shown in Figure 2 were generated and analyzed using Chromas version 2.6.6 software or Geneious R8 version.

Article Snippet: Gene Ruler 1 kb Plus DNA ladder (Thermoscientific, catalog number: SM1333) 18.

Techniques: DNA Sequencing, Amplification, Mutagenesis, Clone Assay, Marker, Generated, Software